Cryo-SEM

Equipment used:

MIRA3 LM

A fully PC controlled FE SEM intended for both – for high vacuum as well as for low vacuum operations. Outstanding optical properties, flicker-free...

TESCAN USA

Low temperature scanning electron microscopy (Cryo-SEM) has become an established technique for capturing and observing biological samples close to their natural state. It is a method of choice, where the traditional sample preparation (e.g. critical point drying) causes unwanted changes in the sample structure. A Cryo-SEM workflow typically involves sample fixation using either a flash-freezing in liquid nitrogen slush or high-pressure freezing. Frozen samples are then transferred under vacuum to a cryo sputter coater, where they are coated with a conductive layer of metals or carbon. Finally, the samples are inserted into a SEM chamber equipped with a cryo-stage and observed under high vacuum condition. Due to our customizable design, all TESCAN SEMs can be equipped with a variety of cryo-processing tools from major vendors. This allows researchers to take advantage of all our imaging capabilities with the state-of-the-art cryo technology of their choice. By integrating cryo SEM in our microscopes, users can take an unprecedented look at nanometer sized features on plants, tissues and cellular specimens.

This article was taken from the website www.tescan.com
 

Surface of a mouse tongue

A cryo-fractured leaf

Application note:

Surface features on cryo-prepared cells.

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TIMA-product_focus

The TESCAN Integrated Mineral Analyzer. TIMA, a fully automated, high throughput, analytical scanning electron microscope is designed specifically for the mining and minerals processing industry. The TIMA solution will address applications such as Mineral Liberation Analysis,TESCAN, a world leading manufacturer of scanning electron microscopes and focused ion beam workstations has introduced the TESCAN Integrated Mineral Analyzer.

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